不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理
2012年10月12日 14:42 来源：农博网
文章摘要： 不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理ACOMPARISONSTUDYOFTHEINVITROANTICANCERACTIVITYANDMECHANISMOFETHANOLEXTRACTSFROMMYCELIAOFDIFFERENTGANODERMALUCIDUMSTRAINS杨晓彤 周月琴 李绪全 冯慧琴 糜可 杨庆尧 摘 �...
不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理
A COMPARISON STUDY OF THE IN VITROANTICANCER ACTIVITY AND MECHANISM OF ETHANOL EXTRACTS FROM MYCELIA OF DIFFERENT GANODERMA LUCIDUM STRAINS
杨晓彤 周月琴 李绪全 冯慧琴 糜可 杨庆尧
摘 要：以人急性早幼粒白血病细胞HL-60细胞株作为模型,用MTT法测定生长抑制率,流式细胞Annexin V实验和DNA含量法研究抑瘤机制,比较了8个灵芝Ganodermalucidum菌株发酵菌丝体乙醇提取物的体外抗肿瘤活性和抑瘤机制.筛选结果得到了能产生高抑瘤活性乙醇提取物的灵芝菌株L5.其对HL-60细胞的抑制率为91.4±0.9%(72小时,125μg/mL);作用48小时后,13.3%的细胞发生早期凋亡,G0/G1期细胞比例比对照组增加15.9%,而S期细胞比例则下降了8.4%,G2/M期细胞数减少7.6%.本研究证明了灵芝菌丝体乙醇提取物能有效抑制HL-60肿瘤细胞的体外生长,抑制率与菌株相关,其抑瘤机制与细胞Go/G1期阻滞和诱导凋亡有关.
关键词：细胞周期,凋亡,HL-60细胞
分类号：Q936 文献标识码：A
文章编号：1672-6472(2005)02-0251-0258
基金项目：上海市教育委员会科学项目资助(项目号04DB20)
作者简介：杨晓彤,通讯作者:email:xtyang@shnu.edu.cn
作者单位：杨晓彤（上海师范大学微生物与免疫学研究所,上海,200234）
周月琴（上海师范大学微生物与免疫学研究所,上海,200234）
李绪全（上海师范大学微生物与免疫学研究所,上海,200234）
冯慧琴（上海师范大学微生物与免疫学研究所,上海,200234）
糜可（上海师范大学微生物与免疫学研究所,上海,200234）
杨庆尧（上海师范大学微生物与免疫学研究所,上海,200234）
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菌物学报
MYCOSYSTEMA
2005 Vol.24 No.2 P.251-258
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